Sweet corn hybrid SEY6RH1263 and parents thereof

ABSTRACT

The invention provides seed and plants of sweet corn hybrid SEY6RH1263 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of sweet corn hybrid SEY6RH1263 and the parent lines thereof, and to methods for producing a sweet corn plant produced by crossing such plants with themselves or with another sweet corn plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the parts of such plants.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of sweet corn hybrid SEY6RH1263 and theinbred sweet corn lines SEY-6RNTB001 and SEY084-SESM1709.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,better stalks, better roots, resistance to insecticides, herbicides,pests, and disease, tolerance to heat and drought, reduced time to cropmaturity, better agronomic quality, higher nutritional value, sugarcontent, uniformity in germination times, stand establishment, growthrate and maturity, among others.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a plant of the sweet cornhybrid designated SEY6RH1263, the sweet corn line SEY-6RNTB001 or sweetcorn line SEY084-SESM1709. Also provided are corn plants having all thephysiological and morphological characteristics of such a plant. Partsof these corn plants are also provided, for example, including pollen,an ovule, and a cell of the plant.

In another aspect of the invention, a plant of sweet corn hybridSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709comprising an added heritable trait is provided. The heritable trait maycomprise a genetic locus that is, for example, a dominant or recessiveallele. In one embodiment of the invention, a plant of sweet corn hybridSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709 isdefined as comprising a single locus conversion. In specific embodimentsof the invention, an added genetic locus confers one or more traits suchas, for example, male sterility, herbicide resistance, insectresistance, resistance to bacterial, fungal, sugar content, nematode orviral disease, and altered fatty acid, phytate or carbohydratemetabolism. In further embodiments, the trait may be conferred by anaturally occurring gene introduced into the genome of a line bybackcrossing, a natural or induced mutation, or a transgene introducedthrough genetic transformation techniques into the plant or a progenitorof any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of sweet corn hybrid SEY6RH1263and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709. The corn seedof the invention may be provided, in one embodiment of the invention, asan essentially homogeneous population of corn seed of sweet corn hybridSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709.Essentially homogeneous populations of seed are generally free fromsubstantial numbers of other seed. Therefore, seed of hybrid SEY6RH1263and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709 may, inparticular embodiments of the invention, be provided forming at leastabout 97% of the total seed, including at least about 98%, 99% or moreof the seed. The seed population may be separately grown to provide anessentially homogeneous population of sweet corn plants designatedSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709.

In yet another aspect of the invention, a tissue culture of regenerablecells of a sweet corn plant of hybrid SEY6RH1263 and/or sweet corn linesSEY-6RNTB001 and SEY084-SESM1709 is provided. The tissue culture willpreferably be capable of regenerating corn plants capable of expressingall of the physiological and morphological characteristics of thestarting plant, and of regenerating plants having substantially the samegenotype as the starting plant. Examples of some of the physiologicaland morphological characteristics of the hybrid SEY6RH1263 and/or sweetcorn lines SEY-6RNTB001 and SEY084-SESM1709 include those traits setforth in the tables herein. The regenerable cells in such tissuecultures may be derived, for example, from embryos, meristematic cells,immature tassels, microspores, pollen, leaves, anthers, roots, roottips, silk, flowers, kernels, ears, cobs, husks, or stalks, or fromcallus or protoplasts derived from those tissues. Still further, thepresent invention provides corn plants regenerated from a tissue cultureof the invention, the plants having all the physiological andmorphological characteristics of hybrid SEY6RH1263 and/or sweet cornlines SEY-6RNTB001 and SEY084-SESM1709.

In still yet another aspect of the invention, processes are provided forproducing corn seeds, plants and parts thereof, which processesgenerally comprise crossing a first parent corn plant with a secondparent corn plant, wherein at least one of the first or second parentcorn plants is a plant of sweet corn line SEY-6RNTB001 or sweet cornline SEY084-SESM1709. These processes may be further exemplified asprocesses for preparing hybrid corn seed or plants, wherein a first cornplant is crossed with a second corn plant of a different, distinctgenotype to provide a hybrid that has, as one of its parents, a plant ofsweet corn line SEY-6RNTB001 or sweet corn line SEY084-SESM1709. Inthese processes, crossing will result in the production of seed. Theseed production occurs regardless of whether the seed is collected ornot.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent corn plant, oftenin proximity so that pollination will occur for example, mediated byinsect vectors. Alternatively, pollen can be transferred manually. Wherethe plant is self-pollinated, pollination may occur without the need fordirect human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent corn plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent corn plants. Yet another step comprisesharvesting the seeds from at least one of the parent corn plants. Theharvested seed can be grown to produce a corn plant or hybrid cornplant.

The present invention also provides the corn seeds and plants producedby a process that comprises crossing a first parent corn plant with asecond parent corn plant, wherein at least one of the first or secondparent corn plants is a plant of sweet corn hybrid SEY6RH1263 and/orsweet corn lines SEY-6RNTB001 and SEY084-SESM1709. In one embodiment ofthe invention, corn seed and plants produced by the process are firstgeneration (F₁) hybrid corn seed and plants produced by crossing a plantin accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridcorn plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid corn plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SEY6RH1263 and/or sweet corn linesSEY-6RNTB001 and SEY084-SESM1709, the method comprising the steps of:(a) preparing a progeny plant derived from hybrid SEY6RH1263 and/orsweet corn lines SEY-6RNTB001 and SEY084-SESM1709, wherein saidpreparing comprises crossing a plant of the hybrid SEY6RH1263 and/orsweet corn lines SEY-6RNTB001 and SEY084-SESM1709 with a second plant;and (b) crossing the progeny plant with itself or a second plant toproduce a seed of a progeny plant of a subsequent generation. In furtherembodiments, the method may additionally comprise: (c) growing a progenyplant of a subsequent generation from said seed of a progeny plant of asubsequent generation and crossing the progeny plant of a subsequentgeneration with itself or a second plant; and repeating the steps for anadditional 3-10 generations to produce a plant derived from hybridSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709. Theplant derived from hybrid SEY6RH1263 and/or sweet corn linesSEY-6RNTB001 and SEY084-SESM1709 may be an inbred line, and theaforementioned repeated crossing steps may be defined as comprisingsufficient inbreeding to produce the inbred line. In the method, it maybe desirable to select particular plants resulting from step (c) forcontinued crossing according to steps (b) and (c). By selecting plantshaving one or more desirable traits, a plant derived from hybridSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709 isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of sweet cornhybrid SEY6RH1263 and/or sweet corn lines SEY-6RNTB001 andSEY084-SESM1709, wherein the plant has been cultivated to maturity, and(b) collecting at least one corn from the plant.

In still yet another aspect of the invention, the genetic complement ofsweet corn hybrid SEY6RH1263 and/or sweet corn lines SEY-6RNTB001 andSEY084-SESM1709 is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a sweet cornplant, or a cell or tissue of that plant. A genetic complement thusrepresents the genetic makeup of a cell, tissue or plant, and a hybridgenetic complement represents the genetic make up of a hybrid cell,tissue or plant. The invention thus provides corn plant cells that havea genetic complement in accordance with the corn plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SEY6RH1263 and/or sweet corn linesSEY-6RNTB001 and SEY084-SESM1709 could be identified by any of the manywell known techniques such as, for example, Simple Sequence LengthPolymorphisms (SSLPs) (Williams et al., 1990), Randomly AmplifiedPolymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF),Sequence Characterized Amplified Regions (SCARs), Arbitrary PrimedPolymerase Chain Reaction (AP-PCR), Amplified Fragment LengthPolymorphisms (AFLPs) (EP 534 858, specifically incorporated herein byreference in its entirety), and Single Nucleotide Polymorphisms (SNPs)(Wang et al., 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by corn plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a corn plant of the invention with a haploid geneticcomplement of a second corn plant, preferably, another, distinct cornplant. In another aspect, the present invention provides a corn plantregenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

In still yet another aspect, the invention provides a method ofdetermining the genotype of a plant of sweet corn hybrid SEY6RH1263and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709 comprisingdetecting in the genome of the plant at least a first polymorphism. Themethod may, in certain embodiments, comprise detecting a plurality ofpolymorphisms in the genome of the plant. The method may furthercomprise storing the results of the step of detecting the plurality ofpolymorphisms on a computer readable medium. The invention furtherprovides a computer readable medium produced by such a method.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of sweet corn hybrid SEY6RH1263, sweet corn lineSEY-6RNTB001 and sweet corn line SEY084-SESM1709. The hybrid SEY6RH1263was produced by the cross of parent lines SEY-6RNTB001 andSEY084-SESM1709. The parent lines show uniformity and stability withinthe limits of environmental influence. By crossing the parent lines,uniform seed hybrid SEY6RH1263 can be obtained.

SEY6RH1263 is a yellow sugary sweet corn hybrid. The hybrid is an earlymaturity variety taking about 1090 heat units from planting to mid-silk.The hybrid has shown high yield and recovery relative to otherprocessing sweet corn hybrids of similar maturity.

The development of sweet corn hybrid SEY6RH1263 and its parent lines issummarized below.

A. Origin and Breeding History of Sweet Corn Hybrid SEY6RH1263

The hybrid SEY6RH1263 was produced from a cross of the lines designatedSEY-6RNTB001 and SEY084-SESM1709. The parent lines are uniform andstable, as is a hybrid therefrom. A small percentage of variants canoccur within commercially acceptable limits for almost anycharacteristic during the course of repeated multiplication. However novariants are expected.

SEY-6RNTB001 is a yellow sweet corn inbred which was selected for goodeating quality, good ear size, and for having the RpG gene that providesresistance to some races of Puccinia polysora (common rust).

The development of parent line SEY-6RNTB001 can be summarized asfollows:

Winter 1999-2000: The inbred line AS403 (a proprietary Seminis inbred)was grown in a Hawaii nursery on Molokai and was crossed to a stockcarrying the RpG allele. This stock was obtained from Dr. Jerald Patakyof the University of Illinois. It was coded Seminis accession B142 andwas an R168 field corn inbred converted to carry the RpG allele. Thisallele was reported by Dr. Art Hooker to have come from PI 163558. AS403was grown in Hawaii nursery row 9803 and B142 was grown in nursery row9764. The nursery was grown by Hawaiian Research Ltd. under a contractwith Seminis.

Summer 2000: The second ear selection of the F1 of AS403 X B142 wasgrown in the Seminis DeForest, Wis. nursery in row 4198 and was crosspollinated with AS403 in nursery row 4197 to make a BC1.

Winter 2000-2001: The first ear selection of the BC1 (AS403 X B142 XAS403) was grown in the Seminis nursery at Melipilla, Chile in row 7580and was cross pollinated with AS403 in nursery row 7579 to make a BC2.

Summer 2001: Inbred 351 (a proprietary Seminis inbred now namedSEY093-351 under the Monsanto naming) was grown in Seminis DeForest,Wis. nursery in row 4366. This inbred was crossed with a rust resistantplant from the 9^(th) ear selection of the BC2 {[(AS403 X B 142) XAS403] X AS403} grown in row 3847.

Winter 2001-2002: The above cross with inbred 351 was grown in theSeminis Melipilla, Chile nursery in row 6818 and a rust resistant plantin row 6818 was cross pollinated with inbred 351 in row 6817 to make aBC1 to inbred 351.

Summer 2002: The 2^(nd) ear selection of the BC1 to inbred 351 was grownin the Seminis DeForest, Wis. nursery in row 5185 and a rust resistantplant in row 5185 was crossed with inbred 351 in row 5183 to make a BC2to inbred 351.

Winter 2002-2003: The BC2 to inbred 351 was grown in the SeminisMelipilla, Chile nursery in row 8173 and self pollinated to give F2ears.

Summer 2003: F2 seed of the ear 1 selection was grown Seminis DeForest,Wis. nursery row 5175 and rust resistant plants were pollinated.

Winter 2003-2004: F3 seed of the ear 1 selection was grown in SeminisMelipilla, Chile nursery row 4979 and self pollinated.

Summer 2004: F4 seed of the ear 1 selection was grown in SeminisDeForest, Wis. nursery row 3968 and rust resistant plants were selfpollinated.

Winter 2004-2005: F5 seed of the ear 1 selection was grown in SeminisMelipilla, Chile nursery row 8502 and self pollinated.

Summer 2005: F6 seed of the 2^(nd) ear selection was grown in SeminisDeForest, Wis. nursery row 5859 and self pollinated. Ear 1 was selectedand given the name designation SEY-6RNTB001.

Corn inbred SEY-6RNTB001 was named at the BC2F7 generation. This inbredwas reproduced by self pollination in 2009-2010 in Melipilla, Chilewinter nursery and judged to be stable. Fifty ear selections from the2009 Chile increase were sent to Foundation Seed as Breeder's Seed.Inbred SEY-6RNTB001 is uniform for all traits observed.

SEY084-SESM1709 is an early yellow sweet corn inbred which was selectedfor good eating quality, resistance to Maize Dwarf Mosaic Virus (MDMV),Sugarcane Mosaic Virus (SCMV) and the Rp1D gene which providesresistance to some races of Puccinia sorghi (common rust).

The development of parent line SEY084-SESM1709 can be summarized asfollows:

Summer 1996: The inbred line SEPN 2 (a proprietary Seminis inbred) wasgrown in the DeForest, Wis. nursery in row 3644 and was crossed by aplant in row 4321 of the nursery which had shown MDMV resistance andwhich carried the Rp1D gene. This stock in row 4321 was an S3 line buttesting vs MDMV and common rust had shown it homozygous resistant toboth pathogens. This F1 cross was given a source of N96: 3644×4321/1

Winter 1997-1998: The F1 was grown in Homestead, Fla. nursery row 228and was cross pollinated with SEPN 2 (a proprietary Seminis inbred) innursery row 227 to make a BC1. The nursery was grown for Seminis by 27Farms who were paid for their services. The BC1 cross was given a sourceof C97: 228×227/1.

Summer 1997: The BC1 was grown in the Seminis DeForest, Wis. nursery inrow 3761. Plants in the row were inoculated with Sugarcane Mosaic Virus(SCMV) and with Puccinia sorghi. Some of the plants resistant to bothpathogens were selfed. The ear harvested from one of these plants wasgiven the source designation N97: 3761/1*.

Winter 1997-1998: S1 seed of N97: 3761/1* was grown in Rancagua, Chilerow 6208 and selfs were made. The Rancagua nursery was grown for Seminisby Massai Agricultural Services who were paid for their services. One ofthe S2 ears harvested was given the source designation E98: 6208/1*.

Summer 1998: S2 seed of E98: 6208/1* was grown ear-to-row in the SeminisDeforest, Wis. nursery in row 5314. Plants in the row were inoculatedwith SCMV and Puccinia sorghi. A plant resistant to both pathogens wasself pollinated. The S3 ear harvested form that plant was given thesource designation N98: 5314/1*.

Winter 1998-1999: S3 seed of N98: 5314/1* was grown ear-to-row in Hawaiinursery row 8588. Some of the plants in row 8588 were self pollinated.The nursery was grown for Seminis by Hawaiian Research Ltd. Who werepaid for their services. One of the selfed ears harvested from row 8588was given the source designation H99: 8588/1*.

Summer 1999: S4 seed of H99: 8588/1* was grown ear-to-row in DeForest,Wis. nursery in row 4240. Plants in the row were inoculated withSugarcane Mosaic Virus (SCMV) and with Puccinia sorghi. All 17 plants inthe row were resistant to MDMV but not all plants were resistant toPuccinia sorghi. Some of the plants resistant to both pathogens wereselfed. The ear harvested from one of these plants was given the sourcedesignation N99: 4240/1*.

Winter 1999-2000: S5 seed of N99: 4240/1* was grown ear-to-row in row7053 of the Seminis Melipilla, Chile nursery. Some plants were selfpollinated. One of the selfed ears harvested from row 7053 was given thesource designation E00: 7053/1*. This ear was also given the namedesignation N1195EQ9.

Summer 2000: S6 seed of E00: 7053/1* was grown ear-to-row in DeForest,Wis. nursery in row 3430. Plants in the row were inoculated withSugarcane Mosaic Virus (SCMV) and with Puccinia sorghi. All 20 plants inthe row were resistant to MDMV and were resistant to Puccinia sorghirace 0. Some of the plants were selfed. The ear harvested from one ofthese plants was given the source designation N00: 3430/1*.

Winter 2000-2001: S7 seed of N00: 3430/1*was grown ear-to-row in row7988 of the Seminis Melipilla, Chile nursery. Some plants were selfpollinated. Eight of the selfed ears were harvested and shelled seedfrom those ears was bulked into a single bag of seed. This bulk wasgiven the source designation E01: 7988/B1*

Summer 2001: S8 seed of E01: 7988/B1* was grown ear-to-row in row 7146of the Seminis Nampa, Id. nursery. All plants were observed foruniformity and self pollinated. All self pollinated ears were harvestedand shelled seed from those ears was bulked into a single bag of seed.This bulk was given the source designation A01: 7146/*. This stock wasgiven the name SESM1709. Later when Seminis naming policy changed theline was renamed SEY084-SESM1709.

Summer 2002: S9 seed of A01: 7146/* was grown in row 5057 of the SeminisNampa, Id. nursery. All plants were observed for uniformity and selfpollinated. All self pollinated ears were harvested and shelled seedfrom those ears was bulked into a single bag of seed. This bulk wasgiven the source designation A02: 50571*.

Summer 2004: S10 seed of A02: 50571* was grown in row 9049 of theSeminis Nampa, Id. nursery. All plants were observed for uniformity andself pollinated. One of the pollinated ears was given a sourcedesignation of A04: 9049/12*.

Winter 2004-2005: S11 seed of A04: 9049/12* was planted in the DeForest,Wis. greenhouse experiment 2004-6 and all plants were inoculated withboth SCMV and Puccinia sorghi race 0. All 38 plants tested wereresistant to both pathogens. Seed of A04: 9049/12* was also grown in row6832 of the Melipilla, Chile nursery. Plants were observed foruniformity and uniform ears were selected at harvest. All seed savedfrom this row was bulked as the first Foundation Seed increase for thisinbred. This seed was given the source designation FSCC6382/05.

Corn inbred SEY084-SESM1709 was named at the BC1S9 generation. Thisinbred was reproduced at the BC1S11 by self pollination in the Winter2004-2005 Melipilla, Chile winter nursery and judged to be stable. Abulk of ear selections from the 2004-2005 Chile increase made up thefirst Foundation Seed lot. Inbred SEY084-SESM1709 is uniform for alltraits observed.

B. Physiological and Morphological Characteristics of Sweet Corn HybridSEY6RH1263, Sweet Corn Line SEY-6RNTB001 and Sweet Corn LineSEY084-SESM1709

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of sweet corn hybrid SEY6RH1263 and the parent linesthereof. A description of the physiological and morphologicalcharacteristics of such plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSEY6RH1263 Comparison Variety- Characteristic SEY6RH1263 GH 2042 1. Typesweet sweet 2. Region where developed in the midwest midwest U.S.A. 3.Leaf foliage: intensity of green color medium medium first leaf:anthocyanin absent or very weak medium coloration of sheath first leaf:shape of apex pointed pointed leaf: undulation of margin of blade absentor very weak intermediate leaf: angle between blade and small (±25°)very small stem (on leaf just above upper ear) leaf: curvature of bladeslightly recurved moderately recurved leaf: anthocyanin coloration ofsheath absent or very weak absent or very weak leaf: width of blade widemedium width of ear node leaf 8.8 cm 8.43 cm standard deviation:standard deviation: 0.35051 0.7286 sample size: 15 sample size: 15length of ear node leaf in 72.36 cm 79.33 cm centimeters standarddeviation: standard deviation: 2.3335 3.3946 sample size: 15 samplesize: 15 number of leaves above top 5.2 4.6 ear standard deviation:standard deviation 0.5606 0.5071 sample size: 15 sample size: 15 degreesleaf angle 40° 40° color 5GY 4/4 5GY 4/4 sheath pubescence (1 = none to9 8 9 = like peach fuzz) marginal waves (1 = none to 1 7 9 = many)longitudinal creases (1 = none 8 6 to 9 = many) stem: degree of zig-zagabsent or very slight slight stem: anthocyanin coloration absent or veryweak absent or very weak of brace roots stem: anthocyanin colorationabsent or very weak absent or very weak of internodes 4. Plant length(tassel included) (only medium long inbred lines and varieties with eartype of grain: sweet or pop) ratio height of insertion of small largepeduncle of upper ear to plant length peduncle: length short long plantheight (to tassel tip) 118.6 cm 121.33 cm standard deviation: standarddeviation 7.2806 8.7723 sample size: 15 sample size: 30 ear height (tobase of top ear 29.3 cm 34.26 cm node) standard deviation: standarddeviation 3.3209 7.6107 sample size: 15 sample size: 15 length of topear internode 12.94 cm 18.03 cm standard deviation: standard deviation:1.3201 2.5471 sample size: 15 sample size: 15 average number of tillers3.2 avg 2.1 avg standard deviation: standard deviation 0.7745 0.4577sample size: 15 sample size: 15 average number of ears per 1.8 avg 1.6avg stalk standard deviation: standard deviation: 0.41403 0.5071 samplesize: 15 sample size: 15 anthocyanin of brace roots absent absent 5.Tassel time of anthesis very early early anthocyanin coloration at baseabsent or very weak absent or very weak of glume anthocyanin colorationof absent or very weak absent or very weak glumes excluding baseanthocyanin coloration of absent or very weak absent or very weakanthers angle between main axis and small (±25°) small lateral branchescurvature of lateral branches absent or very slightly absent or veryslightly recurved recurved number of primary lateral many few branchesdensity of spikelets moderately dense medium length of main axis abovemedium long lowest lateral branch (A-B) length of main axis above mediummedium highest lateral branch (C-D) length of lateral branch shortmedium number of primary lateral 33.93 24.1 branches standard deviation:standard deviation: 4.4315 4.7278 sample size: 15 sample size: 15 branchangle from central 27.66° 41° spike standard deviation: standarddeviation: 10.9978 8.981 sample size: 15 sample size: 15 length (fromtop leaf collar to 34.33 cm 37.23 cm tassel tip) standard deviation:standard deviation: 2.0845 2.7894 sample size: 15 sample size: 15 pollenshed (0 = male sterile to 9 7 9 = heavy shed) anther color 2.5R 7/42.5GY 7/4 glume color 2.5GY 8/2 5GY 7/4 bar glumes (glume bands) presentabsent 6. Ear (unhusked data) silk color 2.5GY 8/6 2.5GY 8/4 (unhuskeddata) fresh husk 5GY 7/4 5GY 6/6 color (unhusked data) dry husk 2.5Y 8/62.5Y 8/4 color (unhusked data) position of horizontal horizontal ear atdry husk stage (unhusked data) husk 2 5 tightness (1 = very loose to 9 =very tight) (unhusked data) husk short (ears exposed) medium extension(at harvest) (husked ear data) ear length 17.8 cm 17.66 cm standarddeviation: standard deviation: 2.2819 1.3047 sample size: 15 samplesize: 15 (husked ear data) ear diameter 50.3 mm 43.7 mm at mid-pointstandard deviation: standard deviation: 9.1255 1.4959 sample size: 15sample size: 15 (husked ear data) ear weight 162.3 gm 132 gm standarddeviation: standard deviation: 24.5754 16.8607 sample size: 15 samplesize: 15 (husked ear data) number of 20.2 17.4 kernel rows standarddeviation: standard deviation: 2.2103 1.4041 sample size: 15 samplesize: 15 (husked ear data) kernel rows indistinct distinct (husked eardata) row straight slightly curved alignment (husked ear data) shanklength 15.2 cm 16.4 cm standard deviation: standard deviation: 2.68464.8309 sample size: 15 sample size: 15 (husked ear data) ear taperslight distinct length long long diameter (in middle) large large shapeconico-cylindrical conico-cylindrical number of rows of grain mediummedium number of colors of grains one one (only varieties with ear typeof grain: sweet or waxy) grain: intensity of yellow medium dark color(only varieties with ear type of grain: sweet) grain: length (onlyvarieties long long with ear type of grain: sweet) grain: width (onlyvarieties broad medium with ear type of grain: sweet) type of grainsweet sweet shrinkage of top of grain (only strong medium varieties withear type of grain: sweet) color of top of grain yellow yellowanthocyanin coloration of absent or very weak absent or very weak glumesof cob time of silk emergence (50% very early early of plants)anthocyanin coloration of absent or very weak absent or very weak silks7. Cob diameter of mid-point 28.1 mm 24.3 mm standard deviation:standard deviation: 2.7117 1.1139 sample size: 15 sample size: 15 color2.5Y 8/4 2.5Y 8/2 8. Kernel (dried) length 12.5 mm 10.8 mm standarddeviation: standard deviation: 0.5637 0.6453 sample size: 15 samplesize: 15 width 7.4 mm 6.8 mm standard deviation: standard deviation:1.109 0.9259 sample size: 15 sample size: 15 thickness 3.2 mm 2.9 mmstandard deviation: standard deviation 0.5398 0.5767 sample size: 15sample size: 15 % round kernels (shape grade) sample size: 15 samplesize: 15 aleurone color pattern homozygous homozygous aleurone color2.5Y 8/10 2.5Y 8/10 hard endosperm color 2.5Y 8/10 2.5Y 8/10 endospermtype sweet (su1) sweet weight per 100 kernels 18 gm 23 gm (unsizedsample) sample size: 100 sample size: 100 9. Agronomic Traits stay green(at 65 days after 3 3 anthesis) (from 1 = worst to 9 = excellent)dropped ears (at 65 days after 26.60% 50% anthesis) *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of Sweet CornLine SEY-6RNTB001 Comparison Variety- Characteristic SEY-6RNTB001 WE10 1. Type sweet sweet 2. Region where developed in the midwest midwestU.S.A. 3. Leaf foliage: intensity of green color medium dark first leaf:anthocyanin absent or very weak absent or very weak coloration of sheathfirst leaf: shape of apex pointed pointed to round leaf: undulation ofmargin of blade intermediate intermediate leaf: angle between blade andlarge (±75°) small stem (on leaf just above upper ear) leaf: curvatureof blade slightly recurved strongly recurved leaf: anthocyanincoloration of sheath absent or very weak absent or very weak leaf: widthof blade medium narrow width of ear node leaf 7.26 cm 7.48 cm standarddeviation: standard deviation: 0.4169 0.8067 sample size: 15 samplesize: 45 length of ear node leaf in 59.8 cm 73.77 cm centimetersstandard deviation: standard deviation: 2.56904 3.4635 sample size: 15sample size: 45 number of leaves above top 5.7 6.24 ear standarddeviation: standard deviation 0.7037 0.7075 sample size: 15 sample size:45 degrees leaf angle 70° 30.1° color 5GY 4/6 5GY 3/4 sheath pubescence(1 = none to 9 5 9 = like peach fuzz) marginal waves (1 = none to 5 6 9= many) longitudinal creases (1 = none 2 0 to 9 = many) stem: degree ofzig-zag absent or very slight absent or very slight stem: anthocyanincoloration absent or very weak absent or very weak of brace roots stem:anthocyanin coloration absent or very weak absent or very weak ofinternodes 4. Plant length (tassel included) (only short long inbredlines and varieties with ear type of grain: sweet or pop) ratio heightof insertion of small medium peduncle of upper ear to plant lengthpeduncle: length short long plant height (to tassel tip) 79 cm 91.86 cmstandard deviation: standard deviation 9.1651 7.4712 sample size: 15sample size: 45 ear height (to base of top ear 21.26 cm 29.94 cm node)standard deviation: standard deviation 2.0571 3.2529 sample size: 15sample size: 45 length of top ear internode 8.1 cm 11.6 cm standarddeviation: standard deviation: 0.7121 1.8103 sample size: 15 samplesize: 45 average number of ears per 1.4 avg 1.46 avg stalk standarddeviation: standard deviation: 0.5071 0.5768 sample size: 15 samplesize: 45 anthocyanin of brace roots absent absent 5. Tassel time ofanthesis early early anthocyanin coloration at base absent or very weakabsent or very weak of glume anthocyanin coloration of weak absent orvery weak glumes excluding base anthocyanin coloration of absent or veryweak absent or very weak anthers angle between main axis and very small(<5°) very small lateral branches curvature of lateral branches absentor very slightly straight recurved number of primary lateral many mediumbranches density of spikelets medium medium length of main axis aboveshort medium lowest lateral branch (A-B) length of main axis above veryshort short highest lateral branch (C-D) length of lateral branch shortmedium number of primary lateral 20.5 33.7 branches standard deviation:standard deviation: 6.5559 3.9892 sample size: 15 sample size: 45 branchangle from central 22.66° 34.7° spike standard deviation: standarddeviation: 5.6273 9.1999 sample size: 15 sample size: 45 length (fromtop leaf collar to 19.86 cm 29.78 cm tassel tip) standard deviation:standard deviation: 1.7368 3.1981 sample size: 15 sample size: 45 pollenshed (0 = male sterile to 9 8 9 = heavy shed) anther color 2.5Y 8/62.5GY 8/10 glume color 2.5GY 7/4 5GY 6/6 bar glumes (glume bands)present absent 6. Ear (unhusked data) silk color 2.5GY 8/4 25GY 8/6(unhusked data) fresh husk 2.5GY 6/8 2.5GY 6/8 color (unhusked data) dryhusk 5Y 8/4 2.5Y 8/4 color (unhusked data) position of horizontalupright ear at dry husk stage (unhusked data) husk 3 6 tightness (1 =very loose to 9 = very tight) (unhusked data) husk long (8-10 cm beyondmedium extension (at harvest) the ear tip) (husked ear data) ear length13.4 cm 15.1 cm standard deviation: standard deviation: 1.1153 2.0126sample size: 15 sample size: 45 (husked ear data) ear diameter 38.1 mm37.02 mm at mid-point standard deviation: standard deviation: 5.67944.4629 sample size: 15 sample size: 45 (husked ear data) ear weight 37.6gm 76.8 gm standard deviation: standard deviation: 12.6923 26.3475sample size: 15 sample size: 45 (husked ear data) number of 12 15.8kernel rows standard deviation: standard deviation: 2.9032 3.0608 samplesize: 15 sample size: 45 (husked ear data) kernel rows indistinctdistinct (husked ear data) row straight straight alignment (husked eardata) shank length 9.8 cm 11.14 cm standard deviation: standarddeviation: 2.4886 3.0598 sample size: 15 sample size: 45 (husked eardata) ear taper extreme slight length medium medium diameter (in middle)large medium shape conico-cylindrical conico-cylindrical number of rowsof grain medium medium number of colors of grains one one (onlyvarieties with ear type of grain: sweet or waxy) grain: intensity ofyellow light dark color (only varieties with ear type of grain: sweet)grain: length (only varieties medium medium with ear type of grain:sweet) grain: width (only varieties broad medium with ear type of grain:sweet) type of grain sweet sweet shrinkage of top of grain (only mediummedium varieties with ear type of grain: sweet) color of top of grainyellow yellow anthocyanin coloration of absent or very weak absentglumes of cob time of silk emergence (50% early late to very late ofplants) anthocyanin coloration of absent or very weak absent silks 7.Cob diameter of mid-point 27.4 mm 27.65 mm standard deviation: standarddeviation: 1.8798 2.1848 sample size: 15 sample size: 45 color 2.5Y 8/25Y 8/4 8. Kernel (dried) length 8.3 mm 7.05 mm standard deviation:standard deviation: 1.5668 0.9999 sample size: 15 sample size: 45 width7.9 mm 6.75 mm standard deviation: standard deviation: 1.4203 0.8364sample size: 15 sample size: 45 thickness 2.5 mm 5 mm standarddeviation: standard deviation 0.6725 0.9935 sample size: 15 sample size:45 % round kernels (shape grade) sample size: 15 sample size: 45aleurone color pattern homozygous homozygous aleurone color 5Y 8/10 2.5Y8/10 hard endosperm color 5Y 8/10 2.5Y 8/10 endosperm type sweet (su1)sweet weight per 100 kernels 18 gm 16.25 gm (unsized sample) samplesize: 100 sample size: 200 9. Agronomic Traits stay green (at 65 daysafter 1 7 anthesis) (from 1 = worst to 9 = excellent) *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.

TABLE 3 Physiological and Morphological Characteristics of Sweet CornLine SEY084-SESM1709 Comparison Variety - Characteristic SEY084-SESM1709 WE 10 1. Type sweet sweet 2. Region where developed in themidwest midwest U.S.A. 3. Leaf foliage: intensity of green color mediumdark first leaf: anthocyanin medium absent or very weak coloration ofsheath first leaf: shape of apex pointed pointed to round leaf:undulation of margin of blade intermediate intermediate leaf: anglebetween blade and small (±25°) small stem (on leaf just above upper ear)leaf: curvature of blade slightly recurved strongly recurved leaf:anthocyanin coloration of sheath weak absent or very weak leaf: width ofblade medium narrow width of ear node leaf 8.5 cm 7.48 cm standarddeviation: standard deviation: 0.9063 0.8067 sample size: 15 samplesize: 45 length of ear node leaf in 57.4 cm 73.77 cm centimetersstandard deviation: standard deviation: 3.6606 3.4635 sample size: 15sample size: 45 number of leaves above top 4.6 6.24 ear standarddeviation: standard deviation 0.7367 0.7075 sample size: 15 sample size:45 degrees leaf angle 52° 30.1° color 5GY 4/6 5GY 3/4 sheath pubescence(1 = none to 9 5 9 = like peach fuzz) marginal waves (1 = none to 7 6 9= many) longitudinal creases (1 = none 1 0 to 9 = many) stem: degree ofzig-zag slight absent or very slight stem: anthocyanin coloration strongabsent or very weak of brace roots stem: anthocyanin coloration weakabsent or very weak of internodes 4. Plant length (tassel included)(only medium long inbred lines and varieties with ear type of grain:sweet or pop) ratio height of insertion of small medium peduncle ofupper ear to plant length peduncle: length very short long plant height(to tassel tip) 89.33 cm 91.86 cm standard deviation: standard deviation10.8671 7.4712 sample size: 15 sample size: 45 ear height (to base oftop ear 25.6 cm 29.94 cm node) standard deviation: standard deviation3.3094 3.2529 sample size: 15 sample size: 45 length of top earinternode 10.73 cm 11.6 cm standard deviation: standard deviation:0.9611 1.8103 sample size: 15 sample size: 45 average number of tillers2.6 avg 2.8 avg standard deviation: standard deviation 0.9102 0.6901sample size: 15 sample size: 45 average number of ears per 1.7 avg 1.46avg stalk standard deviation: standard deviation: 0.4577 0.5768 samplesize: 15 sample size: 45 anthocyanin of brace roots faint absent 5.Tassel time of anthesis very early early anthocyanin coloration at basestrong absent or very weak of glume anthocyanin coloration of strongabsent or very weak glumes excluding base anthocyanin coloration ofstrong absent or very weak anthers angle between main axis and large(±75°) very small lateral branches curvature of lateral branchesmoderately curved straight number of primary lateral few medium branchesdensity of spikelets moderately lax medium length of main axis abovelong medium lowest lateral branch (A-B) length of main axis above verylong short highest lateral branch (C-D) length of lateral branch mediummedium number of primary lateral 15.6 33.7 branches standard deviation:standard deviation: 2.7723 3.9892 sample size: 15 sample size: 45 branchangle from central 69.3° 34.7° spike standard deviation: standarddeviation: 14.3759 9.1999 sample size: 15 sample size: 45 length (fromtop leaf collar to 28.53 cm 29.78 cm tassel tip) standard deviation:standard deviation: 1.6417 3.1981 sample size: 15 sample size: 45 pollenshed (0 = male sterile to 7 8 9 = heavy shed) anther color 2.5R 6/42.5GY 8/10 glume color 2.5R 6/4 5GY 6/6 bar glumes (glume bands) presentabsent 6. Ear (unhusked data) silk color 2.5GY 8/4 2.5GY 8/6 (unhuskeddata) fresh husk 2.5GY 7/4 5GY 6/8 color (unhusked data) dry husk 2.5Y8/4 2.5Y 8/4 color (unhusked data) position of upright upright ear atdry husk stage (unhusked data) husk 3 6 tightness (1 = very loose to 9 =very tight) (unhusked data) husk very long (>10 cm) medium extension (atharvest) (husked ear data) ear length 15.74 cm 15.1 cm standarddeviation: standard deviation: 1.406 2.0126 sample size: 15 sample size:45 (husked ear data) ear diameter 29.94 mm 37.02 mm at mid-pointstandard deviation: standard deviation: 3.0714 4.4629 sample size: 15sample size: 45 (husked ear data) ear weight 24 gm 76.8 gm standarddeviation: standard deviation: 9.5093 26.3475 sample size: 15 samplesize: 45 (husked ear data) number of 6.8 15.8 kernel rows standarddeviation: standard deviation: 2.6956 3.0608 sample size: 15 samplesize: 45 (husked ear data) kernel rows indistinct distinct (husked eardata) row slightly curved straight alignment (husked ear data) shanklength 9.84 cm 11.14 cm standard deviation: standard deviation: 2.09923.0598 sample size: 15 sample size: 45 (husked ear data) ear taperextreme slight length short medium diameter (in middle) small mediumshape conico-cylindrical conico-cylindrical number of rows of grain veryfew medium number of colors of grains one one (only varieties with eartype of grain: sweet or waxy) grain: intensity of yellow medium darkcolor (only varieties with ear type of grain: sweet) grain: length (onlyvarieties short medium with ear type of grain: sweet) grain: width (onlyvarieties broad medium with ear type of grain: sweet) type of grainsweet sweet shrinkage of top of grain (only medium medium varieties withear type of grain: sweet) color of top of grain yellow orange yellowanthocyanin coloration of absent or very weak absent glumes of cob timeof silk emergence (50% very early late to very late of plants)anthocyanin coloration of absent or very weak absent silks 8. Cobdiameter of mid-point 21.5 mm 27.65 mm standard deviation: standarddeviation: 1.3512 2.1848 sample size: 15 sample size: 45 color 5Y 8/42.5Y 8/4 9. Kernel (dried) length 7.38 mm 7.05 mm standard deviation:standard deviation: 1.6234 0.9999 sample size: 15 sample size: 45 width9.48 mm 6.75 mm standard deviation: standard deviation: 0.6885 0.8364sample size: 15 sample size: 45 thickness 4.56 mm 5 mm standarddeviation: standard deviation 0.7393 0.9935 sample size: 15 sample size:45 % round kernels (shape grade) sample size: 15 sample size: 45aleurone color pattern homozygous homozygous aleurone color 5Y 8/12 2.5Y8/10 hard endosperm color 5Y 8/12 2.5Y 8/10 endosperm type sweet (su1)sweet weight per 100 kernels 22 gm 16.25 gm (unsized sample) samplesize: 100 sample size: 200 10. Agronomic Traits stay green (at 65 daysafter 1 7 anthesis) (from 1 = worst to 9 = excellent) *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.

C. Breeding Corn Plants

One aspect of the current invention concerns methods for producing seedof sweet corn hybrid SEY6RH1263 involving crossing sweet corn linesSEY-6RNTB001 and SEY084-SESM1709. Alternatively, in other embodiments ofthe invention, hybrid SEY6RH1263, line SEY-6RNTB001, or lineSEY084-SESM1709 may be crossed with itself or with any second plant.Such methods can be used for propagation of hybrid SEY6RH1263 and/or thesweet corn lines SEY-6RNTB001 and SEY084-SESM1709, or can be used toproduce plants that are derived from hybrid SEY6RH1263 and/or the sweetcorn lines SEY-6RNTB001 and SEY084-SESM1709. Plants derived from hybridSEY6RH1263 and/or the sweet corn lines SEY-6RNTB001 and SEY084-SESM1709may be used, in certain embodiments, for the development of new cornvarieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SEY6RH1263 followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.\

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSEY6RH1263 and/or sweet corn lines SEY-6RNTB001 and SEY084-SESM1709 forthe purpose of developing novel corn lines, it will typically bepreferred to choose those plants which either themselves exhibit one ormore selected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, male sterility, herbicideresistance, resistance for bacterial, fungal, or viral disease, insectresistance, male fertility, sugar content, and enhanced nutritionalquality.

D. Performance Characteristics

As described above, hybrid SEY6RH1263 exhibits desirable agronomictraits. The performance characteristics of hybrid SEY6RH1263 were thesubject of an objective analysis of the performance traits relative toother varieties. The results of the analysis are presented below.

TABLE 4 Performance Data for Hybrid SEY6RH1263 Cases cut Cases cut GreenEar wt corn/acre (@ corn/ton Avg Ear Avg Ear Kernel Row hybrid %Moisture % recovery tons/acre 14.25 lbs/case) green ear Length DiameterNumber SEY6RH1263 70.1 60.0 9.3 781 84 8.38 2.20 18 Average GH 2042 68.150.1 8.7 613 70 8.02 2.00 17.2 Average GH 4927 68.1 46.9 7.9 521 66 8.111.97 17.4 Average 2010 Early Sugary Yellow Processor Sweet Corn YieldTrial - Adams, WI.

TABLE 5 Additional Performance Data for Hybrid SEY6RH1263 Cases cutkernels/ Heat Green ear acre Units to Harvest Wt. (14.25 lbs/ % DESCHarvest Moistue % (lbs/Acre) case) Recovery GH4927 1695 71.8 19,700379.5 27.5 SEY6RH1263 1704 75.0 19,200 510.6 39.4 This record 31 1.73,000 79.2 5.8 contains LSDs for each trait 2010 South Central MinnesotaSugary Yellow Hybrid Yield Trial. 2 planting dates with 3 reps perplanting.

E. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those corn plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalcorn plant which contributes the locus for the desired characteristic istermed the nonrecurrent or donor parent. This terminology refers to thefact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental corn plant to whichthe locus or loci from the nonrecurrent parent are transferred is knownas the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a corn plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny corn plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of corn the recurrent parent as determinedat the 5% significance level when grown in the same environmentalconditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,male sterility, waxy starch, herbicide resistance, resistance forbacterial, fungal, or viral disease, insect resistance, sugar content,male fertility and enhanced nutritional quality. These genes aregenerally inherited through the nucleus, but may be inherited throughthe cytoplasm. Some known exceptions to this are genes for malesterility, some of which are inherited cytoplasmically, but still act asa single locus trait.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of corn plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., 1990), Randomly Amplified Polymorphic DNAs(RAPDs), DNA Amplification Fingerprinting (DAF), Sequence CharacterizedAmplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction(AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., 1998).

F. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., 1985). Moreover, recent technological advances in vectorsfor Agrobacterium-mediated gene transfer have improved the arrangementof genes and restriction sites in the vectors to facilitate theconstruction of vectors capable of expressing various polypeptide codinggenes. The vectors described have convenient multi-linker regionsflanked by a promoter and a polyadenylation site for direct expressionof inserted polypeptide coding genes. Additionally, Agrobacteriumcontaining both armed and disarmed Ti genes can be used fortransformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., 1985; U.S. Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., 1985; Omirulleh et al., 1993; Fromm et al., 1986;Uchimiya et al., 1986; Marcotte et al., 1988). Transformation of plantsand expression of foreign genetic elements is exemplified in Choi et al.(1994), and Ellul et al. (2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., 1985), including in monocots(see, e.g., Dekeyser et al., 1990; Terada and Shimamoto, 1990); atandemly duplicated version of the CaMV 35S promoter, the enhanced 35Spromoter (P-e35S); 1 the nopaline synthase promoter (An et al., 1988);the octopine synthase promoter (Fromm et al., 1989); and the figwortmosaic virus (P-FMV) promoter as described in U.S. Pat. No. 5,378,619and an enhanced version of the FMV promoter (P-eFMV) where the promotersequence of P-FMV is duplicated in tandem; the cauliflower mosaic virus19S promoter; a sugarcane bacilliform virus promoter; a commelina yellowmottle virus promoter; and other plant DNA virus promoters known toexpress in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., 1988), (2)light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., 1989; maize rbcSpromoter, Schaffner and Sheen, 1991; or chlorophyll a/b-binding proteinpromoter, Simpson et al., 1985), (3) hormones, such as abscisic acid(Marcotte et al., 1989), (4) wounding (e.g., wunl, Siebertz et al.,1989); or (5) chemicals such as methyl jasmonate, salicylic acid, orSafener. It may also be advantageous to employ organ-specific promoters(e.g., Roshal et al., 1987; Schernthaner et al., 1988; Bustos et al.,1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a corn plant according to the invention.Non-limiting examples of particular genes and corresponding phenotypesone may choose to introduce into a corn plant include one or more genesfor insect tolerance, such as a Bacillus thuringiensis (B.t.) gene, pesttolerance such as genes for fungal disease control, herbicide tolerancesuch as genes conferring glyphosate tolerance, and genes for qualityimprovements such as yield, nutritional enhancements, environmental orstress tolerances, or any desirable changes in plant physiology, growth,development, morphology or plant product(s). For example, structuralgenes would include any gene that confers insect tolerance including butnot limited to a Bacillus insect control protein gene as described in WO99/31248, herein incorporated by reference in its entirety, U.S. Pat.No. 5,689,052, herein incorporated by reference in its entirety, U.S.Pat. Nos. 5,500,365 and 5,880,275, herein incorporated by reference intheir entirety. In another embodiment, the structural gene can confertolerance to the herbicide glyphosate as conferred by genes including,but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPSgene (aroA:CP4) as described in U.S. Pat. No. 5,633,435, hereinincorporated by reference in its entirety, or glyphosate oxidoreductasegene (GOX) as described in U.S. Pat. No. 5,463,175, herein incorporatedby reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., 1991). The RNA could also be a catalytic RNA molecule (i.e., aribozyme) engineered to cleave a desired endogenous mRNA product (seefor example, Gibson and Shillito, 1997). Thus, any gene which produces aprotein or mRNA which expresses a phenotype or morphology change ofinterest is useful for the practice of the present invention.

G. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a corn varietyare recovered in addition to the characteristics of the single locustransferred into the variety via the backcrossing technique and/or bygenetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a corn plant by transformation.

H. Deposit Information

A deposit of sweet corn hybrid SEY6RH1263 and inbred parent lineSEY-6RNTB001, disclosed above and recited in the claims, has been madewith the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The date of the deposits were Apr. 22,2011 and Apr. 13, 2011, respectively. The accession numbers for thosedeposited seeds of sweet corn hybrid SEY6RH1263 and inbred parent lineSEY-6RNTB001 are ATCC Accession Number PTA-11845 and ATCC AccessionNumber PTA-11815, respectively. Upon issuance of a patent, allrestrictions upon the deposits will be removed, and the deposits areintended to meet all of the requirements of 37 C.F.R. §1.801-1.809. Thedeposits will be maintained in the depository for a period of 30 years,or 5 years after the last request, or for the effective life of thepatent, whichever is longer, and will be replaced if necessary duringthat period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

REFERENCES

The following references, to the extent that they provide exemplaryprocedural or other details supplementary to those set forth herein, arespecifically incorporated herein by reference:

-   U.S. Pat. No. 5,378,619-   U.S. Pat. No. 5,463,175-   U.S. Pat. No. 5,500,365-   U.S. Pat. No. 5,563,055-   U.S. Pat. No. 5,633,435-   U.S. Pat. No. 5,689,052-   U.S. Pat. No. 5,880,275-   An et al., Plant Physiol., 88:547, 1988.-   Bird et al., Biotech. Gen. Engin. Rev., 9:207, 1991.-   Bustos et al., Plant Cell, 1:839, 1989.-   Callis et al., Plant Physiol., 88:965, 1988.-   Choi et al., Plant Cell Rep., 13: 344-348, 1994.-   Dekeyser et al., Plant Cell, 2:591, 1990.-   Ellul et al., Theor. Appl. Genet., 107:462-469, 2003.-   EP 534 858-   Fraley et al., Bio/Technology, 3:629-635, 1985.-   Fromm et al., Nature, 312:791-793, 1986.-   Fromm et al., Plant Cell, 1:977, 1989.-   Gibson and Shillito, Mol. Biotech., 7:125, 1997-   Klee et al., Bio-Technology, 3(7):637-642, 1985.-   Kuhlemeier et al., Plant Cell, 1:471, 1989.-   Marcotte et al., Nature, 335:454, 1988.-   Marcotte et al., Plant Cell, 1:969, 1989.-   Odel et al., Nature, 313:810, 1985.-   Omirulleh et al., Plant Mol. Biol., 21(3):415-428, 1993.-   Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985.-   Roshal et al., EMBO J., 6:1155, 1987.-   Schaffner and Sheen, Plant Cell, 3:997, 1991.-   Schernthaner et al., EMBO J., 7:1249, 1988.-   Siebertz et al., Plant Cell, 1:961, 1989.-   Simpson et al., EMBO J., 4:2723, 1985.-   Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990.-   Uchimiya et al., Mol. Gen. Genet., 204:204, 1986.-   Wang et al., Science, 280:1077-1082, 1998.-   Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990.-   WO 99/31248

What is claimed is:
 1. A corn plant comprising at least a first set ofthe chromosomes of sweet corn line SEY-6RNTB001, a sample of seed ofsaid line having been deposited under ATCC Accession No. PTA-11815.
 2. Aseed comprising at least a first set of the chromosomes of sweet cornline SEY-6RNTB001, a sample of seed of said line having been depositedunder ATCC Accession No. PTA-11815.
 3. The plant of claim 1, which ishybrid.
 4. The plant of claim 3, wherein the hybrid plant is sweet cornhybrid SEY6RH1263, a sample of seed of said hybrid having been depositedunder ATCC Accession No. PTA-11845.
 5. A plant part of the plant ofclaim
 1. 6. The plant part of claim 5, further defined as an ear, ovule,pollen or cell.
 7. The plant part of claim 6, further defined as a cell.8. A corn plant, or a part thereof, having all the physiological andmorphological characteristics of the corn plant of claim
 1. 9. A cornplant, or a part thereof, having all the physiological and morphologicalcharacteristics of the corn plant of claim
 4. 10. A tissue culture ofregenerable cells of the plant of claim
 1. 11. The tissue cultureaccording to claim 10, comprising cells or protoplasts from a plant partselected from the group consisting of leaf, pollen, embryo, root, roottip, anther, silk, flower, kernel, ear, cob, husk, stalk and meristem.12. A corn plant regenerated from the tissue culture of claim
 11. 13. Amethod of vegetatively propagating the plant of claim 1 comprising thesteps of: (a) obtaining tissue capable of being propagated from a plantaccording to claim 1; (b) cultivating said tissue to obtain proliferatedshoots; and (c) rooting said proliferated shoots to obtain rootedplantlets.
 14. A method of introducing a desired trait into a corn linecomprising: (a) crossing a plant of line SEY-6RNTB001, a sample of seedof said line having been deposited under ATCC Accession No. PTA-11815,with a second corn plant that comprises a desired trait to produce F1progeny; (b) selecting an F1 progeny that comprises the desired trait;(c) crossing the selected F1 progeny with a plant of line SEY-6RNTB001to produce backcross progeny; and (d) repeating steps (b) and (c) threeor more times to produce selected fourth or higher backcross progenythat comprise the desired trait.
 15. A corn plant produced by the methodof claim
 14. 16. A method of producing a plant comprising a transgene,the method comprising introducing a transgene into a plant of sweet cornhybrid SEY6RH1263 or sweet corn line SEY-6RNTB001, a sample of seed ofsaid hybrid and line having been deposited under ATCC Accession No.PTA-11845 and ATCC Accession No. PTA-11815, respectively.
 17. A plantproduced by the method of claim
 16. 18. A plant of sweet corn hybridSEY6RH1263 or sweet corn line SEY-6RNTB001 further comprising atransgene, a sample of seed of said hybrid and line having beendeposited under ATCC Accession No. PTA-11845 and ATCC Accession No.PTA-11815, respectively.
 19. A seed that produces the plant of claim 18.20. A plant of sweet corn hybrid SEY6RH1263 or sweet corn lineSEY-6RNTB001 comprising a single locus conversion, a sample of seed ofsaid hybrid and line having been deposited under ATCC Accession No.PTA-11845 and ATCC Accession No. PTA-11815, respectively.
 21. A seedthat produces the plant of claim
 20. 22. A method for producing a seedof a plant derived from hybrid SEY6RH1263 or line SEY-6RNTB001comprising the steps of: (a) crossing a corn plant of hybrid SEY6RH1263or line SEY-6RNTB001 with a second corn plant; a sample of seed of saidhybrid and line having been deposited under ATCC Accession No. PTA-11845and ATCC Accession No. PTA-11815, respectively; and (b) allowing seed ofa hybrid SEY6RH1263 or line SEY-6RNT8001-derived corn plant to form. 23.The method of claim 22, further comprising the steps of: (c) crossing aplant grown from said hybrid SEY6RH1263 or SEY-6RNTB001-derived sweetcorn seed with itself or a second sweet corn plant to yield additionalhybrid SEY6RH1263 or SEY-6RNTB001-derived corn seed; (d) growing saidadditional hybrid SEY6RH1263 or SEY-6RNTB001-derived corn seed of step(c) to yield additional hybrid SEY6RH1263 or SEY-6RNTB001-derived cornplants; and (e) repeating the crossing and growing steps of (c) and (d)to generate at least a first further hybrid SEY6RH 1263 orSEY-6RNTB001-derived corn plant.
 24. The method of claim 22, wherein thesecond corn plant is of an inbred corn line.
 25. A method of producing acorn comprising: (a) obtaining a plant according to claim 1, wherein theplant has been cultivated to maturity; and (b) collecting a corn fromthe plant.
 26. The method of claim 25, wherein the plant is a plant ofsweet corn hybrid SEY6RH1263, a sample of seed of said hybrid SEY6RH1263having been deposited under ATCC Accession No. PTA-11845.
 27. A methodof producing seed comprising crossing the plant of claim 1 with itselfor a second plant.